| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,588,404 | 0 | T | A | 61.1% | ‑2.0 / 18.9 | 18 | Y146F (TAC→TTC) | livM | branched chain amino acid ABC transporter permease LivM |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base A (0/11); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAATCGGCAGGCAGGTCCAGAAGCCC‑AAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTC > NZ_CP009273/3588327‑3588490 | cAGCAATCGGCAGGCAGGTCCAGAAGCCC‑AAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGt > 2:459720/1‑90 (MQ=255) cacAAACGGCCGGGCGGGCCAAAAACCCAAAGCCGAAATAGTGATTGAGCAGCGAAAAAGTGTAAGCGCCGAGGGCGAAAAAACCGCCGt < 2:171014/88‑1 (MQ=255) cc‑AAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGAc > 2:383456/1‑90 (MQ=255) cc‑AAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGAc > 1:62380/1‑90 (MQ=255) cccGTAATAGTGATTGAGCACCGAAAAAGTGAAAGCGCCGAGGGCGAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACcaac < 1:339024/89‑1 (MQ=255) tttttGAGCAGCGAAAAAGTGTAGGCGCCGGGGGTAAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTc < 2:315610/87‑1 (MQ=255) gATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCa > 1:88776/1‑90 (MQ=255) ttGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGc > 2:120475/1‑90 (MQ=255) gcagcGAAAAAGTGTAAGCGCCGAGGGCGAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCga < 1:226208/90‑1 (MQ=255) gcgcAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGAc > 1:75148/1‑90 (MQ=255) gaaaagGTGTAAGCGCCGAGGGCGAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCg < 2:77789/88‑1 (MQ=255) gaaaaaGTGAAAGCGCCGATGGAGAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCg < 2:33037/88‑1 (MQ=255) aaaaGTGAAAGCGCCGAGGGCGAAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAg < 1:291489/90‑1 (MQ=255) aaaaGTGAAAGCGCCGAGGGCAAAAAACCCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAg < 2:88776/90‑1 (MQ=255) gtAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAAt > 2:333551/1‑90 (MQ=255) aGCGCCGAGGGCGAAAAACCCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTa < 2:75148/90‑1 (MQ=255) aGCGCCGAGGGCAAAAAACCCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTa < 2:109526/90‑1 (MQ=255) gCGAAAAACCCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTc < 1:145073/90‑1 (MQ=255) | CAGCAATCGGCAGGCAGGTCCAGAAGCCC‑AAGCCGTAATAGTGATTGAGCAGCGCAAAAGTGTAAGCGCCGATGGCGTAAAAACCGCCGTACCCCAGCACCAGCAGACCAGAAAGACCAACAACCACGTTCAGCCCGAGACCGAGGATAATGTAGATCATGGTC > NZ_CP009273/3588327‑3588490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |