| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,748,723 | 0 | A | C | 62.5% | 7.6 / 14.8 | 16 | G383G (GGT→GGG) | aldB | aldehyde dehydrogenase AldB |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/10); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGTTGAGGATGGTTT > NZ_CP009273/3748636‑3748805 | cGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCAcc > 2:85578/1‑90 (MQ=255) cctcctGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTgc > 1:314334/1‑90 (MQ=255) cacCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTGCGCcgcccgcc > 1:25414/1‑90 (MQ=255) gTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTc > 2:53146/1‑90 (MQ=255) gACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCCCCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAgcgc < 1:416630/90‑1 (MQ=255) gACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAgcgc > 1:251259/1‑90 (MQ=255) ccAAACAAAATCGTCGGTTCGGGGTAGTAGCCTTTTTTCAGTTCCCCTTCCAGCAGCTTGCCCCGCCCGCCTGTGAGCACGTCAGCGccc < 2:189416/90‑1 (MQ=255) caaaaCAGAATCGTCGGTTGGGGGTAGTGGCCGTTTTTCAGTTCCCCTTCCAGCAGCTTGCGCCCCCCGCCTGTGAGCACGTCAGCGccc < 2:396609/88‑1 (MQ=255) gAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCCCCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCttttt < 2:75380/90‑1 (MQ=255) aaaccgtcgGTCGGGGGTAGTGGCCGTCTTTCATTCCCCCTCCCAGCAGCTTGCCCCGCCCGCCTGTGAGCACGTCAGCGCCCTCttttt < 2:401527/86‑1 (MQ=255) gtcgGTTCGAGGTAGTAGCCGTCTTTCAGTTCCCCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCg < 1:179367/90‑1 (MQ=255) ttCGGGGTGGTGGCCGTTTTTCATTTCCCCTCCCAGCACCTTGCCCCCCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATc < 2:251259/90‑1 (MQ=255) agtagCCGTCTTTCAGTTCCCCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGt < 1:364540/90‑1 (MQ=255) gtttttCAGTTCCCCTTCCAGCAGTTTGCCCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGTTGAGGAt < 2:295458/87‑1 (MQ=255) gTCTTTCAGTTCCCCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGTTGAGGAt < 1:85578/90‑1 (MQ=255) tCAGTTCACCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGTTGAGGATGGttt < 2:25414/90‑1 (MQ=255) | CGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCAGCAGCTTGCGCCGCCCGCCTGTGAGCACGTCAGCGCCCTCTTTTTTACCGATATCAATGTAGTTGAGGATGGTTT > NZ_CP009273/3748636‑3748805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |