Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,775,3710AC58.3% 9.4 / 12.5 12G184G (GGT→GGG) cysEserine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (0/7);  total (2/10)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGCCCGCGCCAATCATCACACCTTCACGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGTGGTCAAG  >  NZ_CP009273/3775305‑3775457
                                                                  |                                                                                      
cgcgcccgcgccAATCATCACACCTTCACGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAGATTGCAg                                                                 <  2:7384/90‑1 (MQ=255)
   gcccgcgccAATCATCACACCTTCACGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAGATTGCAGAAt                                                              <  2:397568/90‑1 (MQ=255)
                 tcaCACCTTCACGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGtttt                                                >  2:21235/1‑90 (MQ=255)
                           aCGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAg                                                                        >  1:132711/1‑56 (MQ=255)
                           aCGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAg                                                                        <  2:132711/56‑1 (MQ=255)
                                      ggTGACGGTCACCCCCAGATTTCCCCGTCCCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCaa                           <  1:201317/90‑1 (MQ=255)
                                                accaccAGATTTCCCCGTCCCGCCAAGCGTCACAGATTGCAGAATCGATCCGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGcc                 <  2:161181/90‑1 (MQ=255)
                                                        aTTTCCCCGTCCCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGt         <  1:216672/90‑1 (MQ=255)
                                                        aTTTCCCCGTCCCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGt         <  2:70467/90‑1 (MQ=255)
                                                             cccGTCCCGCCAGGCGTCACAGATTGAAGAATCGATACGTCGTTTTAAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGTGGTCa    <  2:441184/90‑1 (MQ=255)
                                                             cccGTCCCGCCAAGCGCCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGTGGTCa    <  1:303525/90‑1 (MQ=255)
                                                               cGTCCCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGTGGTCAAg  <  1:195735/90‑1 (MQ=255)
                                                                  |                                                                                      
CGCGCCCGCGCCAATCATCACACCTTCACGAATTTTCGGGTGACGGTCACCACCAGATTTACCCGTACCGCCAAGCGTCACAGATTGCAGAATCGATACGTCGTTTTCAATCACCGCCGTTTCACCAACGACGATGCCTGTCGCGTGGTCAAG  >  NZ_CP009273/3775305‑3775457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑