| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,926 | 0 | A | C | 54.5% | 5.1 / 11.4 | 11 | G219G (GGT→GGG) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAT > NZ_CP009273/4527841‑4528005 | aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCtt < 1:73501/90‑1 (MQ=255) gTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAg > 2:87591/1‑90 (MQ=255) taaaaTCCGGCACCCTTTCGCAACCCTGTTTTGGCTCCCCCATT‑AATAAGCCAGT‑TTTTATCCCCTTTATCCACAACCGCCGCACCAGcc < 1:42085/88‑1 (MQ=255) gCACCCGTTCGCAATCCTGTTTTGGCTTCCCCATT‑AAAAAGCCAGG‑TTTTATCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTAc < 1:164421/90‑1 (MQ=255) gTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGc > 2:333670/1‑90 (MQ=255) aaccTTGTTTTGCTTTCCCATTAAAAAAGCCGGT‑TTTTACCCCTTTTTTTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCg < 1:418608/88‑1 (MQ=255) tcttgTTTGGCCTCCCCAAT‑AAAAAACCCGGTTTTTACCCCTTTTATCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 1:444352/86‑1 (MQ=255) ggCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCATTCCGTACCAGGGCGATTCGCCAGCGTAACt > 2:399446/1‑90 (MQ=255) ccttt‑AAAAAGCGGGT‑TTTTACCCCTTTTATACAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc < 1:206118/87‑1 (MQ=255) ccATT‑AATAAGCCAGG‑TTTTATCCCCTTTTTTCACAACCCCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc < 2:127535/90‑1 (MQ=255) aGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt > 2:113539/1‑90 (MQ=255) | AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAT > NZ_CP009273/4527841‑4528005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |