| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,612,067 | 0 | A | G | 56.5% | 7.2 / 22.7 | 23 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base A (3/7); new base G (13/0); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCA > NZ_CP009273/4611991‑4612152 | ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAg > 1:103888/1‑90 (MQ=255)ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATTAg > 2:376047/1‑90 (MQ=255) tctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGc < 2:293711/90‑1 (MQ=255) tctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGc < 2:279592/90‑1 (MQ=255) ctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTTGTCGGGGGGGGGGATTATCaagg > 2:212214/1‑87 (MQ=255) ctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATCAGCg > 1:248262/1‑90 (MQ=255) ctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGTGGTTGGGGGGGGGGGGGGATTATTAGCg > 2:264706/1‑90 (MQ=255) ggAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGAt < 1:216787/90‑1 (MQ=255) aagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTg < 1:376047/90‑1 (MQ=255) aTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGGGGGGGGGGGGGGGGATTTTCAGAGGTGGAATAATGccc > 2:229180/1‑88 (MQ=255) gCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCacaaca > 1:123445/1‑90 (MQ=255) ctctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGAATATTAGCGATGGGTTACTGCCCAaaac > 2:257724/1‑90 (MQ=255) tGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGgaa > 1:165180/1‑90 (MQ=255) tGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAAAACCCCGCGGgaa > 2:81911/1‑90 (MQ=255) gAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCTGCGGAAATTCCTCTTGATCaaaaa > 1:418103/1‑90 (MQ=255) aCTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCAcc < 2:288766/90‑1 (MQ=255) aCTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCAcc < 2:3007/90‑1 (MQ=255) cTGGCGGTTGGGTGGGGGGGGGGGGGAAATATCAGCGATGGATTACTGCACAACACCCCGCGGGAATTCCTCTGGGTCAAAAAATCAccc > 2:451920/1‑90 (MQ=255) tGGCGGTTGGTTGGGCGGGGGGGGGGAATATTAGCGAGGGGTTACTGCACAAAACCCTGCGGGAAATTCTCTGGATCAAAAACTCAcccc > 2:320992/1‑89 (MQ=255) ggCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATTGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAAATCACCCAt > 1:59346/1‑90 (MQ=255) gCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCAtt > 1:293184/1‑90 (MQ=255) ggttggttGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTAc > 1:17585/1‑90 (MQ=255) ggttggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAAc < 1:320992/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 2:206953/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 1:29076/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 1:257724/90‑1 (MQ=255) | CTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCA > NZ_CP009273/4611991‑4612152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |