| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 842,900 | 0 | G | T | 53.3% | 17.4 / 26.1 | 15 | I187I (ATC→ATA) | glnH | glutamine ABC transporter substrate‑binding protein GlnH |
| Reads supporting (aligned to +/- strand): ref base G (0/7); new base T (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGAT > NZ_CP009273/842815‑842980 | cgCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTtgatga < 1:409961/90‑1 (MQ=255) gTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTACAGAATGTTTTGCGGATCGTg > 1:224437/1‑90 (MQ=255) gctgcGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGGAGGAAAGAAAGTTTTGGGGATCGTGGAGaa > 2:3534/1‑90 (MQ=255) gctgcGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGGTTTTTTGAAAAAAAGAAAGTTTTGCGGATCGGGCAGaa > 2:663389/1‑90 (MQ=255) agagTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGcgcg < 2:316532/90‑1 (MQ=255) cACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTgg < 2:28183/90‑1 (MQ=255) cTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGc < 1:667235/90‑1 (MQ=255) aCCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGccc < 1:458784/90‑1 (MQ=255) aCCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGccc < 2:190279/90‑1 (MQ=255) gCTTTGAACTGACCGTTACCGGCGGTTTTTTTTGAGTACAGAAAGTTTTTCGGATCCTGCAGAACGGGGGCTGCGCGGGTGGGGGCCAGt > 2:413514/1‑90 (MQ=255) tttGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTc < 1:33421/90‑1 (MQ=255) tGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAg < 1:92224/90‑1 (MQ=255) gACCGTTACCGGCGGTTTTTTTGAAGTACAGAAAGTTTTGGGGATCGTGGGGAACGGGGGCTGGGCGGGTGGTGGCCCGTTTCCtataag > 2:667972/1‑88 (MQ=255) gACCGTTACCGGCGGTTTTTATGAAGGACAGAATGTTTGGCGGATCGTGCAGAAAGGCGGCTGCGCGGGTGGGGCCCCGGTCCATATAgg > 1:204070/1‑90 (MQ=255) ttACCGGCGGTTTTTTTGAAATACAGAATGTTTGGCGTATCGTGCAGAACGGCGGCTGCGCGGGTGGGGCCCCGTTCCCTATAGGCGTTa > 2:40962/1‑90 (MQ=255) ggcggTTTTTATGAAGTACAGAATGTTTGGCGGATCGTGCAGAACGGCGTCTGCGCGGGTGGGGCCCCGCTCCCTATAGGGGGTATTGAt > 1:103714/1‑90 (MQ=255) | CGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGAT > NZ_CP009273/842815‑842980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |