| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,179 | 0 | T | A | 53.3% | 10.9 / 20.7 | 15 | Y72N (TAC→AAC) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base A (8/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT > NZ_CP009273/3401100‑3401258 | tATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAACgggctggg > 1:486997/1‑90 (MQ=255) aTTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACgggctgggc < 1:401772/90‑1 (MQ=255) ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGGGGCTTTTAAAAAAGGGgtgggcgggggggt > 2:367282/1‑86 (MQ=255) cACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCCGGGGCTGCTTTTAAAAACGGGGTGGGGTGGGGATTTGTGGGggtgat > 1:693079/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTTTTAAAAAAGGGTGGGGTGGGGGTTTCTGGGGGTGATTTAGCTTTCTTCAGTCt > 2:202200/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAACGGGGTGGGGTGGGGGTTTCTGGCGGTGATTTAGCTTCCTGCAGTCt > 2:50859/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAAAGGGCTGGGGTGGGGATTTCTGGGGGTGATTTAACTTTCTTCAGTCt > 1:535360/1‑90 (MQ=255) cAGTTCGTTTATCGGCGGGGCCGGGGCTGGTTTTAAAAAAGGGCTGGGCTGGGGTTTGGTGGCGGTGATTTTGCTTTCTGCTggctggct > 2:517341/1‑90 (MQ=255) cAGTTCGTTTATCGGCGGGGCAGGAGGTGCTTATAAAAAAGGGCTGGGGTGGGGATTGCTGGCGGTGGTTTAGCCTCCTTCCGTCTGGCt > 1:510419/1‑90 (MQ=255) tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCgg < 1:192799/90‑1 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCGGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 2:408196/1‑90 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 1:577137/1‑90 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 2:288063/1‑90 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 2:479138/1‑90 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACCCGGTATTCTCGGCAAGAAGtt > 1:179611/1‑90 (MQ=255) | TATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTT > NZ_CP009273/3401100‑3401258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |