| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 5,162 | 0 | T | A | 55.0% | 14.3 / 25.5 | 21 | intergenic (+142/‑72) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (9/0); new base A (0/11); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5073‑5243 | ttttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAAt > 2:709125/1‑90 (MQ=255) gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg > 1:135409/1‑90 (MQ=255) aaaaaTTCCAAAAAAAGGCGGTAATTAAAAAATTAGGATGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcc < 1:793037/88‑1 (MQ=255) gaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAccc > 1:55365/1‑90 (MQ=255) aaaTCCACAATAAATGCGGTAATTAAGAGATTAGGATGGGGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCa < 1:529013/90‑1 (MQ=255) tcAATAATTGCGTAAACTTAGGGATTAGTATTGTTGAAAATAAAAACCGCTGTTCTCATCGAGAAATGTCCGGATATCGACCCATAAccg > 1:682214/1‑88 (MQ=255) tAAAGGCGGTAATTAAGAGATTGGGATTGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 1:325306/90‑1 (MQ=255) aaaaTGCGTTAATTTAGAGATTAGGATTGGGAAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 1:449690/89‑1 (MQ=255) aaaaTGCGTTAATTAAGAGATTAGGATGGCGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 2:250297/89‑1 (MQ=255) aaaaTGCGGTAACTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAGTCTCCGGATATCGACCCATAACGGGCaa < 2:245056/89‑1 (MQ=255) aaggCGGTACTTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATg < 2:135409/87‑1 (MQ=255) gCGGAAACTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATa < 2:418661/90‑1 (MQ=255) ggTAATTTAGAGATTAGGATGGAGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa < 1:709125/90‑1 (MQ=255) ggTAATTAAGAGATTAGGATGGGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa < 2:657178/90‑1 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATTATAAAAGGAGTAACCtgt > 1:765427/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 2:860780/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 2:402085/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 1:784818/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 1:460066/1‑90 (MQ=255) tAGGATGGGGAAAAACAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg < 1:857917/90‑1 (MQ=255) ttggCGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaa < 2:435511/87‑1 (MQ=255) gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa > 2:756677/1‑90 (MQ=255) | TTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5073‑5243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |