| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,636,048 | 0 | T | G | 61.5% | 10.2 / 29.2 | 26 | A52A (GCA→GCC) | rodZ | cytoskeleton protein RodZ |
| Reads supporting (aligned to +/- strand): ref base T (0/10); new base G (16/0); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAG > NZ_CP009273/2635963‑2636122 | gcagcaGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCt < 2:221900/90‑1 (MQ=255) cttctGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCaa < 2:512396/90‑1 (MQ=255) atGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑c < 1:101889/90‑1 (MQ=255) atGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑c < 2:705196/90‑1 (MQ=255) atGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTTTATGTTGCGGA‑c > 2:768112/1‑90 (MQ=255) cAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGCCCTTTTTTTTTTTATGTCTCGTA‑CCGC‑ggg > 2:695022/1‑89 (MQ=255) gACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCCTATTTTTTTTCATGTTGCGTA‑CCGG‑GGgaa > 1:834643/1‑90 (MQ=255) cgcgCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCt < 2:836421/58‑1 (MQ=255) cgcgCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTCTTCTTCAATGTCGCGGA‑CCCT‑GGgaaac > 2:101889/1‑88 (MQ=255) cgcgCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGCCt > 1:836421/1‑58 (MQ=255) cgcATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGCCTTTTCTTTTTTAATTTTGCGGA‑CCGTGGGAAACCt > 2:332946/1‑90 (MQ=255) ggATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTTAATTTTGCGGA‑CCGG‑GGGAAACCTCAGGGaaaaac > 1:334493/1‑87 (MQ=255) gATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTTTATTTCGCGGACCCGG‑GGAAACC‑TCAAGCaaaaacg > 2:721918/1‑86 (MQ=255) gATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTCTATTTCGCGTA‑CCGT‑GGGAAACTTCAGGCaaaaacg > 1:99353/1‑86 (MQ=255) gATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTCCATTTTGCGGACCCGG‑GGAAACC‑TCCGGCaaaaacg > 1:284198/1‑86 (MQ=255) atCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCCTTTCTTTTTTTAATTTGCGTA‑CCGC‑GGGAAACTTTAGGCAAAAACGCTccg > 1:390008/1‑88 (MQ=255) gcgcAGGAATGTTGAAGCAAGATCGGCGGGGGGCGTTTTTTCTTTTATGTCGCGTG‑CCGC‑GGGAACCCTCAGGCAAAAACGCTCGGCCAc > 1:381648/1‑90 (MQ=255) gcgcAGGAATGTTGAAGCAAGATCGGCGGGGGCGTTTTTTTCTTCTATGTCGCGTA‑CCCC‑GGGAACCCTCCAGCAAAAACGGTCGGCCAc > 2:622897/1‑90 (MQ=255) gcAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACgg < 2:390008/90‑1 (MQ=255) aaTGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCctgc < 2:678310/90‑1 (MQ=255) gTTGAAGCAAGATCGGCGGGGGGCTTTTTTTTTTTCATTTTCCGGA‑CCGG‑GGGAAACCTCAGGGAAAAACGCTCCGCAACCGGCCGCTGa > 1:116634/1‑90 (MQ=255) gAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTa < 2:337881/90‑1 (MQ=255) gAAGCAAGATCGGCGGGGGGCTTATCTTCTTCAATATCGCGTA‑CCGC‑GGAAAACCTCAGGCAAAAACGCTCGGCAACCGCCCGCTGGCTa > 1:316356/1‑90 (MQ=255) aaGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTaa < 2:118041/90‑1 (MQ=255) aaGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTaa < 2:785406/90‑1 (MQ=255) aGCAAGATCGGCGGGGGGCTTTTCTTTTTTAAATTCGCGTA‑CCCC‑GGGAAACCTCCGGCAAAATCGCCCGGCAACGGCCCGCTGGCTaaa > 1:118041/1‑89 (MQ=255) | GCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGTGCCTTATCTTCTTCAATGTCGCGTA‑CCGT‑GGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAG > NZ_CP009273/2635963‑2636122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |