| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,946 | 0 | C | G | 55.0% | 5.6 / 17.6 | 20 | A13G (GCA→GGA) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base C (5/4); new base G (11/0); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCG > NZ_CP009273/3175866‑3176031 | tttGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACggt > 2:371220/1‑88 (MQ=255) gACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGttttt > 1:112007/1‑88 (MQ=255) cTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGttt < 2:45373/90‑1 (MQ=255) gTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGCTTTTTTGGGCGGTTCTCTGc > 1:708790/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGCGCGGTTTTCGCC‑gg > 1:485475/1‑89 (MQ=255) ttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑gg < 1:474619/90‑1 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGCGCCCCGTTTTTTTGCGCGTTTTTCCGC‑GGTCTCC‑GGGCaaaa > 2:378442/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGTTTTTTTGCGCGGTTTTCTGC‑GGTTT‑CTGGGGAAAAAc > 1:739163/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGCGCGTTTTTCGGC‑GTTCTCC‑GGGCAAAAAc > 1:2002/1‑90 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGTGCGTTTTTTTGC‑GTTCT‑T‑TGGCAAAAAACCCCCAAcc > 1:695125/1‑90 (MQ=255) tACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTAc < 1:668297/90‑1 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGGTTTTCGGC‑GGTCT‑C‑CGGCAAAAAACCTCCCACCCCTTTCTTGc > 2:406684/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑GGGCAAAAAACCTCGAACCGCTTACTggg > 2:216772/1‑89 (MQ=255) tGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGGTTTTCCGCGGTTTC‑C‑GGGCAAAAACCC‑CCAACCCCCTTCTGGCGTTTTTGc > 1:719552/1‑90 (MQ=255) gACCATACTGGCGGGGGGCGCCACCTTTTTTTGCGCGTTTTTCGGC‑CTTCT‑CGGGGCAAAAACCC‑CCAACCGCCTACTTGGGGTTTTGCt > 1:498894/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCCCGGTTGTTTGCGGGTTTTTCGTC‑GTTTT‑T‑GGGGGAAAAACCCCGAACCCCGTACTGTCGGTTTTGCt > 1:559156/1‑90 (MQ=255) aTACTGGCGGGGGCAGCCACGGTTTTTTGGGCGTTTTTCGGC‑GTTCT‑C‑GGGCAAAAAACCTCGAACCGCTTACTGGCGGTTTTGCTAGGt > 2:869848/1‑90 (MQ=255) tACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTTTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTTGCTAGGtt > 2:253466/1‑90 (MQ=255) gCGGGGGCAGCCACGGTTTTTGGCGCGTTTTTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTTCTGGCGGTTTTGCTAGGGTTTtcg > 2:203756/1‑90 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:48535/90‑1 (MQ=255) | TTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGC‑GTTCT‑C‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCG > NZ_CP009273/3175866‑3176031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |