| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,348,801 | 0 | G | A | 58.8% | ‑1.1 / 14.6 | 17 | E240K (GAA→AAA) | gltB | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base A (0/10); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCAC > NZ_CP009273/3348744‑3348884 | aaCACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATgg > 2:833190/1‑90 (MQ=255)aaCACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATgg > 2:308114/1‑90 (MQ=255) ccGTCCCGCGCGGCCCGCGGGCCCACCCGTCCCCCTTTCTGGCGCATAACGGTAAAACCAACCCCATCCCGGGTAACCGCCAATGGgcgc < 2:744072/90‑1 (MQ=255) cttACCGCGCTGGCCGCTGGCGAAACCGTTCCGCTATCTGGCGCATAACCGGGAAATCAACACCATCACCGGTAGCCGCCAATGTgcgcg > 1:422636/3‑90 (MQ=255) cGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcg > 1:790840/1‑90 (MQ=255) cGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcg > 2:352555/1‑90 (MQ=255) gTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgt > 2:354672/1‑90 (MQ=255) cgcgggccgcgggcGCACCCGTTCCGCTATCGGGCGCATAAGGGTAAAATAAACACCATCCCGGGTAACCGCCAATGGgcgcgtgcgcgt < 1:325261/90‑1 (MQ=255) cggcccgctgccGCACCCGTTCCGCTTTCGGGCGAATAACGGTAAAACCAACACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTAc < 2:82368/86‑1 (MQ=255) ctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATaaa > 1:122165/1‑90 (MQ=255) cgggcGCACCCGTCCCGCTTCCGGGCGCAAAAGGGTAAAATCAACACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATaaa < 1:354672/88‑1 (MQ=255) aCCGTCCCCTTATCGGGCGAAAAGCGGTAAAATCAACCCCATCACCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGac < 1:399857/90‑1 (MQ=255) cGTCCCGCTATCTGGCGCATAACGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGacac < 2:357942/90‑1 (MQ=255) tCTGGCGCATAACGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 2:14230/90‑1 (MQ=255) agcGGTAAAACCACCCCCACCCCCGTTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:806403/88‑1 (MQ=255) accGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:122165/88‑1 (MQ=255) aagggTAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:212776/87‑1 (MQ=255) | AACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCAC > NZ_CP009273/3348744‑3348884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |