| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 58.3% | 21.1 / 27.3 | 24 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (14/0); total (16/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGA > NZ_CP009273/4290890‑4291063 | ggggCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgt > 2:850493/1‑90 (MQ=255) gTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTctct > 2:168886/1‑90 (MQ=255) gTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGctct > 2:420361/1‑90 (MQ=255) tCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCtt > 2:525745/1‑90 (MQ=255) gTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCtttcag > 2:643277/1‑86 (MQ=255) cTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCg < 1:90760/90‑1 (MQ=255) cTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCg < 2:227363/90‑1 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTTAAGCggtt > 2:112871/1‑87 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAGCGGTa > 2:419951/1‑90 (MQ=255) gggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTgag < 2:352808/90‑1 (MQ=255) ggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTgagg < 2:57019/90‑1 (MQ=255) gCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGc < 2:853705/90‑1 (MQ=255) cTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGAcc < 1:419951/90‑1 (MQ=255) ttAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAg < 1:524680/90‑1 (MQ=255) aataaGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGGTATTTTAGGAGAACGGGTGAGTGCGGGTGCCCGACCGGCAGCg > 2:129860/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTTGGGGGAACGGCTGAGGGGGGGTGCCAGACCGGCAGCGGAttt > 2:524680/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTTAGGAGAACGGCTGGGGGGGGGTGCCAGAACGGCAGCGGAttt > 2:760003/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGGGAACGGGTGGGGGCGGGTGGCCGAACGGGAGCGGAttt > 2:446250/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGGTATTTTAGGAGAAAGGCTGAGGGGGGGTGCCAGACCGGCAGCGGAttt > 2:76719/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAGCGTTATTTGAGGAGAAAGGCTGAGTGCGGGTGCCAGACCGGCAGCGGAttt > 2:625188/1‑90 (MQ=255) ggATGGCCTGAGAGGCGGGGGGCTCTTCAAGCGGTATTTTAGGAGAACGGCTGAGTGCGGGTGCCAGACCGGCAGCGGATTTTTCCAggg > 2:420557/1‑90 (MQ=255) gATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGc < 1:521513/90‑1 (MQ=255) aTGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTTTTTTAGGGGAACGGCTGAGGGCGGGTGCCAGACCGGCAGCGGATTTCTCCCGGGCg > 2:514435/1‑90 (MQ=255) aTGGCCTGAGAGGCGGGGGGCTCTTTAAACGGTATTTTAGGGGAAAGGCTGAGGGCGGGTGCCAGACCGGCAGCGTATTTTTCCAggggg > 2:569432/1‑88 (MQ=255) gCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTTTCCAGGGCGTCGGCAAACTGa > 2:206441/1‑90 (MQ=255) | GGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGA > NZ_CP009273/4290890‑4291063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |