Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273700,9960CA54.5% 4.9 / 13.4 11P533T (CCG→ACG) nagEPTS N‑acetyl glucosamine transporter subunit IIABC
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGA  >  NZ_CP009273/700912‑701081
                                                                                    |                                                                                     
aTTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACa                                                                                  >  1:577082/1‑90 (MQ=255)
         gATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAtc                                                                         <  1:164707/90‑1 (MQ=255)
                     cTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCa                                                             <  1:283146/90‑1 (MQ=255)
                                  cTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACGACAGAAAAAAAAGTCGGATCACCCCCCGCCGGGGCaa                                                >  2:417842/1‑90 (MQ=255)
                                           cATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGTCAATCGTGaaaa                                       <  2:493092/90‑1 (MQ=255)
                                                            gtgggtgACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCAc                      <  1:173713/90‑1 (MQ=255)
                                                             tgggtgACGGTGTGGCGGGGAAAACGAAAGAAAAAAAAGGCGTGTCACCCCCCGCCGGGGGAAAAGCGAAAAAATTCAAAACCAACCAcc                     >  2:562900/1‑89 (MQ=255)
                                                                   aCGGTGTGGCGGTGAAAACCACAGAAAAAAACGGGGTAACACCCGGCGCAGGGGCAAAAGGGGAAAACTTCTACAACAACCACGGGGTCt               >  2:512908/1‑90 (MQ=255)
                                                                      gtgtGGCGGTGAAAACGACAGATAAAATCGTCGTATCACCAGCCGCAGGGGCAATCGTGAAAATCTTCAACACCCACCACGCGTTCTGcc            >  1:389322/1‑90 (MQ=255)
                                                                      gtgtGGCGGTGAAAACGACAGAAAAAAACGTCGTATCACCAGCCCCCGGGGCAATCGGGAAAAACTTCAAAACCAACCACCCGGTCTGcc            >  2:173713/1‑90 (MQ=255)
                                                                                gAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGa  >  1:177667/1‑90 (MQ=255)
                                                                                    |                                                                                     
ATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGA  >  NZ_CP009273/700912‑701081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑