| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 57.1% | 8.2 / 14.9 | 14 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAAC > NZ_CP009273/822897‑823047 | aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGTtcgtcg > 1:153419/1‑90 (MQ=255) ccGTGACGCCTTTCTTTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGaa < 2:683145/90‑1 (MQ=255) cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa < 2:92844/90‑1 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAGAATGTc > 2:57346/1‑90 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGACGGGTCGGCGAGAAAAAGAAAGTCCGGGTCAt > 2:112106/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGGGAAAAAGAATGTCCGGGTCAttc > 1:145684/1‑88 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGGAACGCCGGGAGACGGTTGGTCGAGAAACAAAAAAGCCGGGTGATGCATCAACg > 1:498705/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGAGAGGGGGGCAACACCGGAAGTCGGGTTGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGaaa > 1:19886/1‑89 (MQ=255) aGCCAAAATTCACGGCGGGTGAGGGGGGGAACCCCGGAAAGGGGTTCGGGCGGAAAAAAAATGGCCGGGGGCTGCATCCGCGCACAAgcc > 2:479261/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGGAGTCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAGgcc > 1:502978/1‑90 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:577756/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:98467/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:502978/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 1:18572/90‑1 (MQ=255) ggggggCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAAGCCAGCGCCAGCCGCTGTTTAAAAc > 2:377721/1‑90 (MQ=255) | ATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAAC > NZ_CP009273/822897‑823047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |