Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	970,180	0	C	G	55.6%	4.4 / 10.7	9	L136V (CTC→GTC)	mukF	chromosome partition protein MukF
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base G (5/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAA  >  NZ_CP009273/970108‑970238                                                                         |                                                           gACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGAt                                           <  1:300561/90‑1 (MQ=255) gACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGGCGAAAGCGCAGCAGaa                                           >  1:687257/1‑89 (MQ=255)           tCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGTCCAAAACGCCGCAGAAgccgcccgcg                                 >  1:91269/1‑86 (MQ=255)             cGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGGCCAACGCGCGGCCGAAGCCGCCCGagag                               >  1:14769/1‑90 (MQ=255)             cGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGGCCAAAACGCCGCAGAAGGCGCCGCagag                               >  2:69047/1‑90 (MQ=255)                cAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGGGGGGTCTAACACGCCGCCGAAACCGCCGGAGAgggg                            >  2:189077/1‑89 (MQ=255)                                cGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACt            <  2:687257/90‑1 (MQ=255)                                   tGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGc         <  2:315614/90‑1 (MQ=255)                                          ttCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTaa  <  2:15942/90‑1 (MQ=255)                                                                         |                                                           GACTACTACATCCGTCAGCGCGAGTTTTCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCAGATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAA  >  NZ_CP009273/970108‑970238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑