| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,429,984 | 0 | G | A | 57.1% | ‑3.4 / 14.6 | 14 | intergenic (‑108/+33) | uspF/ompN | universal stress protein UspF/porin OmpN |
| Reads supporting (aligned to +/- strand): ref base G (6/0); new base A (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAG > NZ_CP009273/1429897‑1430060 | ccGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 1:382909/1‑90 (MQ=255)ccGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 1:553002/1‑90 (MQ=255) aaGTGAAACCCAAGATGACTTTTTTTGTGTAATGACGGAGTTCACTTTTTTAATTTAGATAAAAGGAGGAAAAATAAGAAAAAAAAGccc < 2:41075/90‑1 (MQ=255) aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATa > 1:199366/1‑90 (MQ=255) aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATa > 1:513593/1‑90 (MQ=255) gATGACTTTTTTTGTGTAATGACGGAGTTCACTTTTTTAATTTAGAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCgg < 1:577806/90‑1 (MQ=255) ctttttttGTGTAATGAGGGATTTCACTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCagg < 1:644779/87‑1 (MQ=255) ttttttGTGAAAGGAGGGATTTCATTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggag < 1:354040/89‑1 (MQ=255) aaaTGAGGGATTTCATTTTTTTAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa < 1:444476/89‑1 (MQ=255) aaaTGACGGAGTTCAATTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGaa < 1:692240/89‑1 (MQ=255) aaTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAAc > 1:324062/1‑90 (MQ=255) aaTTTTAAATTTAGAAAAAAGGAGAAAAAAAAAAAAAAAAACGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc < 2:646297/89‑1 (MQ=255) tttAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa < 1:458486/90‑1 (MQ=255) ggaAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag > 2:312407/1‑90 (MQ=255) | CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAG > NZ_CP009273/1429897‑1430060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |