| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,558,373 | 0 | C | T | 56.2% | 4.1 / 15.8 | 16 | E540K (GAG→AAG) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base T (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGT > NZ_CP009273/1558317‑1558460 | tttCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTgaagaa > 2:314399/1‑90 (MQ=255) ggTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGc > 1:123838/1‑75 (MQ=255) ggTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGc < 2:123838/75‑1 (MQ=255) gCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTc < 1:142331/90‑1 (MQ=255) cttcttTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCt > 2:503661/1‑90 (MQ=255) tttCGGCGCGGCGCTAAAACCCAACCGCTTTTTTCCCTTTTCGTTCACCGCCGGGCTGAAGAC‑CTGCCAGCCGTTACCGCCATTCTTGCg < 2:562078/90‑1 (MQ=255) tttCACCGCGGCGCCAAAAACCAACCGCTTTTTTCCCTTTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCg < 2:184012/90‑1 (MQ=255) cTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAAt > 1:494854/1‑90 (MQ=255) gcgcCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATcc > 2:648000/1‑90 (MQ=255) caaaaaCCAACCCCTTTTTTCCCTTTTCGTTCATCGCCGGGCTGAAGAC‑CGGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattg < 2:582171/88‑1 (MQ=255) ccGCTTTTTTCCCTTTTCTTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 2:136388/89‑1 (MQ=255) aCGCTTTTTTCCCTTTTCTTTCATCGCGGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 2:24461/90‑1 (MQ=255) aCGCTTTTTTCCCTTTTCTTTCATCG‑CGGGGTGAAAAACCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 2:346207/90‑1 (MQ=255) aCGCTTTTTTCCCTTTTCTTTCATCCCGGGGCTAAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 2:530320/90‑1 (MQ=255) aCGCTTTTTTCCCATTTCGTTCACCGCCGGGCTGAAAAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 1:681019/90‑1 (MQ=255) aCGCTTTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGc < 1:26340/90‑1 (MQ=255) ctctTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGt > 1:72526/1‑90 (MQ=255) | TTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAA‑CTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGT > NZ_CP009273/1558317‑1558460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |