| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,634,789 | 0 | A | C | 53.8% | 10.4 / 14.3 | 13 | G18G (GGT→GGG) | essD | phage lysis protein EssD |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGT > NZ_CP009273/1634715‑1634878 | cccAGCCCACCGATTGCACCCCCCTGGGAGGGATTTATTTTATCGAGAAACTGAAATGCAAAAAACCCACCATTCCCCGCCGATGTGCCa < 2:472002/90‑1 (MQ=255) accccGATTGCACCCCACTGTGACGGATTTACTTTATCGAGTAACTGCAATGCCCAAAAACCAGCATTCCCCGCCGATGTGCCATAGGCa < 2:240203/87‑1 (MQ=255) ccGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCAAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAaca > 1:472002/1‑90 (MQ=255) cGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAacac > 2:670677/1‑90 (MQ=255) tttATCGAGTAACTGCAATGCCCAAAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCat < 1:38074/90‑1 (MQ=255) cGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCt > 1:154687/1‑90 (MQ=255) aaCTGCAATGCCCAAAAACCAGCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCAccc < 1:573905/90‑1 (MQ=255) aaCTGCAATCCCCAAAAACCACCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCAccc < 2:5546/90‑1 (MQ=37) aaCTGAAATCCCAAAAAACCAGAATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCAccc < 2:100036/90‑1 (MQ=25) tgaaaTGCCCAGAACCCAGCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGa < 1:670677/87‑1 (MQ=37) cAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGc > 2:403902/1‑90 (MQ=255) aGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCa > 2:531978/1‑90 (MQ=255) aaTTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAat < 2:523350/89‑1 (MQ=255) aCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGt > 1:48404/1‑90 (MQ=255) | CCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGT > NZ_CP009273/1634715‑1634878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |