Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,319,9290TG66.7% ‑2.3 / 17.9 21G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/6);  new base G (14/0);  total (15/6)
Fisher's exact test for biased strand distribution p-value = 1.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA  >  NZ_CP009273/2319844‑2320017
                                                                                     |                                                                                        
aaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATgg                                                                                      <  1:143880/90‑1 (MQ=255)
                  ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                                    <  1:345400/90‑1 (MQ=255)
                        gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGTTTTGcccccccct                                                              >  2:345400/1‑86 (MQ=255)
                        gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGTTTTGcccccccct                                                              >  2:295324/1‑86 (MQ=255)
                          gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCcactta                                                            >  1:265620/1‑90 (MQ=255)
                                       cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAAAATGAGTTTAGCCCCCCACTTTCTCGATGCaaaaa                                               >  2:293879/1‑89 (MQ=255)
                                            gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                          <  2:651424/90‑1 (MQ=255)
                                              cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                        <  1:295324/90‑1 (MQ=255)
                                                ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTg                                      >  1:625510/1‑90 (MQ=255)
                                                        ggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTTCTCGGTGCAAAAGCACGCTCTGGGTATTGt                              >  2:452501/1‑90 (MQ=255)
                                                         gTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTAGTTTATCCCCCCACTTACTCGATGCCAAAGCACGGCCTGGGTATCGtg                             >  1:353856/1‑89 (MQ=255)
                                                         gTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                             >  1:272606/1‑90 (MQ=255)
                                                             ggCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGGTTTGCCCCCCCCTTACTCGATGCAAAAAGACGCTCTGGGTATTGTCTTgg                         >  1:477501/1‑90 (MQ=255)
                                                             ggCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTgg                         >  1:143652/1‑90 (MQ=255)
                                                             ggCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTgg                         >  1:565026/1‑90 (MQ=255)
                                                                gcAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGgaga                      >  1:380262/1‑90 (MQ=255)
                                                                     gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc                 <  1:503589/90‑1 (MQ=255)
                                                                     gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc                 <  2:565026/90‑1 (MQ=255)
                                                                       aTTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGGGACGGGCAg               >  1:691717/1‑90 (MQ=255)
                                                                               ggggggGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTGGGGACGGACAGGGTTATGa       >  2:24426/1‑90 (MQ=255)
                                                                                    gTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGGCGGACAGGTTTATGACGTaa  >  1:166485/1‑90 (MQ=255)
                                                                                     |                                                                                        
AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA  >  NZ_CP009273/2319844‑2320017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑