| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,436,061 | 0 | T | G | 60.0% | 7.2 / 14.2 | 15 | G273G (GGT→GGG) | mnmC | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGC > NZ_CP009273/2435979‑2436126 | aGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTccc > 1:652735/1‑89 (MQ=255) cTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGgtgttg > 2:29576/1‑90 (MQ=255) tGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTg < 1:29576/90‑1 (MQ=255) gCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTCCAGCGCGGTGTTGTTGCTgg > 1:499004/1‑90 (MQ=255) gCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTGCCCGCGCGGTGTTGTTGCTgg > 1:437735/1‑90 (MQ=255) gCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGTTGTTTTTGCTgg > 1:649026/1‑90 (MQ=255) cTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGgc < 1:54903/90‑1 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGGTTGCCCGCGCGTTGTTGTTGCTGGCGCTa > 2:87991/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCCGCGCGGTGTTGTTGCTGGCGCTa > 2:54903/1‑90 (MQ=255) gCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTCCCGCGCGGTGTTTTTGCTGGGGCCATTTCCGGGCGGGTggg > 2:133094/1‑89 (MQ=255) aCGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGGTGGCAg > 1:433371/1‑90 (MQ=255) aCGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTCCAGCGCGGTGTTGTCGCTGGCGCTATTTCGGCGCGGGTGGGAg > 1:622286/1‑90 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 1:697565/90‑1 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 2:499004/90‑1 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 2:652735/90‑1 (MQ=255) | AGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGC > NZ_CP009273/2435979‑2436126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |