Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,020,3000AC54.5% 7.5 / 10.7 11E391D (GAA→GAC) guaDguanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAA  >  NZ_CP009273/3020212‑3020360
                                                                                        |                                                            
ccACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAAc                                                             <  1:651292/90‑1 (MQ=255)
   cGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCa                                                          >  1:687111/1‑90 (MQ=255)
   cGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCa                                                          >  2:222125/1‑90 (MQ=255)
          cggAGCAAAATCTCTGGGCCTTGACGTTTTGTTTGCCAATTTTTTCCCGGGAAAAGAGGCTGTTTTCGTGGTGATGGACCCCACCGCCAc                                                   <  2:683500/90‑1 (MQ=255)
                aaaaTCTCGGGGCTTTGACGTTTTGATTGGCAACTTTTTCCCTGGAAAAGGGGCTGTTTTGGTGGTGATGGACCCCACCGCCACTCCGCt                                             <  1:459732/89‑1 (MQ=255)
                         gggCCTTGACGTTTTGTTGGGCAATTTTTTCCCGGGAAAAGAGGCTGTTTTCGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCt                                    <  2:205032/90‑1 (MQ=255)
                                    ttttGTTGGGCATTTTTTTCCCTGCAAAAGAGGCTGTTTTCGTGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACa                         <  2:99014/89‑1 (MQ=255)
                                    ttttGATTGCCACTTTTTTCCCGGGAAAAGGGGCTTTTTTCGGGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACa                         <  2:376819/89‑1 (MQ=255)
                                          ttGGCAATTTTTTACCGGACAAAGAGGCTGATTTCGTGGTGATGAAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTg                   <  1:441176/90‑1 (MQ=255)
                                                    tttCCCTGGAAAAGGGGTTGTTTTCGGGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGt         <  1:619347/90‑1 (MQ=255)
                                                           ggCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACaaa  >  2:697070/1‑90 (MQ=255)
                                                                                        |                                                            
CCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAA  >  NZ_CP009273/3020212‑3020360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑