Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,905	T→G	L28R (CTC→CGC)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,905	0	T	G	100.0%	124.1 / NA	38	L28R (CTC→CGC)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/25);  total (13/25)

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991                                                                                      |                                                                                       tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  2:499978/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:66944/90‑1 (MQ=255)   aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTg                                                                                  <  1:116252/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  2:183549/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:425752/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  2:154324/1‑90 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTCTCGGCTTGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:424133/90‑1 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  2:120336/90‑1 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     <  1:82725/90‑1 (MQ=255)                    cggcgTTAGCGGGAGAGCGCGTTATCGGCATGAAAAACGCCACGCCGTGGAGCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt                                                                 <  2:493086/90‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:447816/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:336217/1‑90 (MQ=255)                         ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:12848/80‑1 (MQ=255)                         ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  2:12848/1‑80 (MQ=255)                           aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaataa                                                          <  1:159752/90‑1 (MQ=255)                                tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      >  2:183529/1‑73 (MQ=255)                                tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:183529/73‑1 (MQ=255)                                tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                                     <  2:31929/90‑1 (MQ=255)                                 aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  1:210064/90‑1 (MQ=255)                                  gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                                   >  1:599318/1‑90 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTg                                                                                  >  1:449275/1‑55 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTg                                                                                  <  2:449275/55‑1 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                                      >  2:18419/1‑83 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                                      <  1:18419/83‑1 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  1:154932/1‑90 (MQ=255)                                           tccGGCAGGGAAAACGCCATGCCGCGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGgc                                          <  2:599318/88‑1 (MQ=255)                                           accGGCAGGGAAACCGCCATCCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAAGAAACCCGTGATTATGGgc                                          <  2:324979/88‑1 (MQ=255)                                                cATGAAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                     <  2:381632/90‑1 (MQ=255)                                                 aTGGAAACCGCCAGGCCGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  2:468122/90‑1 (MQ=255)                                                 aTGGAAACCCCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  1:422368/90‑1 (MQ=255)                                                     aaaaCGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                >  1:509479/1‑90 (MQ=255)                                                     aaaaCGCCATGCCGGGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                <  2:454904/90‑1 (MQ=255)                                                                  gTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                   >  1:670584/1‑90 (MQ=255)                                                                    ggAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  2:48390/90‑1 (MQ=255)                                                                          tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt           >  2:327639/1‑90 (MQ=255)                                                                            cctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg         >  2:575572/1‑90 (MQ=255)                                                                                   aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  1:27081/90‑1 (MQ=255)                                                                                   aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:44861/90‑1 (MQ=255)                                                                                      |                                                                                       TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49820‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑