Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,776,510	A→G	L171P (CTG→CCG)	gpsA ←	NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,776,510	0	A	G	100.0%	25.0 / NA	9	L171P (CTG→CCG)	gpsA	NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

TGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTG  >  NZ_CP009273/3776424‑3776579                                                                                       |                                                                      tGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcggca                                                                    <  2:383356/90‑1 (MQ=255)     aaTAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGGAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAgctg                                                                >  2:127720/1‑90 (MQ=255)         aCGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTgg                                                            >  2:71729/1‑90 (MQ=255)                    cgcgCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGc                                                 <  2:179681/90‑1 (MQ=255)                      cgccgcCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCaa                                               <  2:47601/90‑1 (MQ=255)                                             aaaTCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGc                        <  1:192369/90‑1 (MQ=255)                                             aaaTCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGc                        <  2:231017/90‑1 (MQ=255)                                                        gCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGt             >  2:399520/1‑90 (MQ=255)                                                             aaaCGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTa        >  2:268144/1‑90 (MQ=255)                                                                   ggAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTg  >  1:325351/1‑90 (MQ=255)                                                                                       |                                                                      TGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTG  >  NZ_CP009273/3776424‑3776579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑