| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,678,659 | 0 | A | C | 57.1% | 8.9 / 16.8 | 14 | Y70D (TAT→GAT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCG > NZ_CP009273/2678570‑2678745 | ggAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATa > 2:502547/1‑90 (MQ=255) cGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCgt > 1:17699/1‑89 (MQ=255) cGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGc > 1:109169/1‑90 (MQ=255) tAGCGTAGTCAGCGCCGAACATTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATAAACATCCTCGCAACCGCCGTAGTAGCGtt < 1:222911/90‑1 (MQ=255) aGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCg > 1:263557/1‑90 (MQ=255) aGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCg > 1:464024/1‑90 (MQ=255) ccgcgcCAAACTTTTCTTCCGCACGACCGATCGCCTTTTTTTAAACAATACCAACATCCCCGAACCCGCCGTAGTAGCGTTTGCCCGGAt < 2:268410/88‑1 (MQ=255) cgcgcCAAACATTTCTTCCGCCCGATCGACCGCCTTTTTTCCAAAAATAACAACATCCCCCCACCCGCCGTAGTAGCGTTTGCCCGGATa < 2:464024/89‑1 (MQ=255) gcgcCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTCCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATaa > 2:266289/1‑90 (MQ=255) gAACATTTCTTTCGCACGATCGATCGCCAGTTTTTCAACGATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 1:276803/90‑1 (MQ=255) gTTCTTTCCCACAATCGATCGCCAGTTGTTAAACAATAACAAAATCCGCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCat < 2:7610/90‑1 (MQ=255) cgatcgccCGCCATTTGTTCAAAAATACCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:413765/90‑1 (MQ=255) cgatcgatCGCCAGTTTTTCAACAATATCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:417526/90‑1 (MQ=255) aCATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTAcgcgc < 1:251244/90‑1 (MQ=255) cATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTAcgcgcg > 1:28425/1‑90 (MQ=255) aCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc > 2:544328/1‑56 (MQ=255) | GGAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCG > NZ_CP009273/2678570‑2678745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |