| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,794,496 | 0 | T | G | 61.5% | 6.4 / 11.2 | 13 | V58G (GTG→GGG) | nrdI | class Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (8/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTG > NZ_CP009273/2794411‑2794585 | cTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCa > 2:389476/1‑90 (MQ=255) ggATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCGACAGGGAATTTGCt > 2:87614/1‑90 (MQ=255) cAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAAATTGCTTTTTa > 2:143126/1‑90 (MQ=255) gCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCGACAGGGAATTTGCCTTTTTAACGACGAGca > 2:52518/1‑90 (MQ=255) gCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGCTGGGGCGGGGCCCCCACAGGGAAATTCCTTTTTTAAAGACGAgga > 2:388733/1‑88 (MQ=255) cTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCCCGACAGGGAATTTGCTTTTTTAACGACGAGCACAAACGGGCg > 2:189090/1‑90 (MQ=255) aTCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTg < 1:270119/90‑1 (MQ=255) gTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCCCGACAGGGAAATTGCTTTTTTAAAGGAGAGCACAAACGGGCGGTGCtt > 2:254177/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcg > 1:398108/1‑90 (MQ=255) tctTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcggc < 2:79572/90‑1 (MQ=255) ggTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGt > 1:452201/1‑90 (MQ=255) gCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGc < 1:232773/90‑1 (MQ=255) gCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGc < 1:389476/90‑1 (MQ=255) tGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTg > 2:247765/1‑90 (MQ=255) | CTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTG > NZ_CP009273/2794411‑2794585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |