| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 63.6% | ‑1.7 / 12.5 | 11 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base A (0/7); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCA > NZ_CP009273/3214029‑3214206 | aTTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGcc > 1:275179/1‑90 (MQ=255) ttgttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGa > 2:146429/1‑90 (MQ=255) aaaTCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAc > 1:374593/1‑90 (MQ=255) aTCGGTTATATCTTTGCCAGGGAGATTTCCCGCCGGCGCGTACGGGGGGCCGAAACGCTAAAAACCGCAAAAACGACCCGCATTGAACCg < 2:275179/90‑1 (MQ=255) ttataACTTTGCCAGGGAGATGCCCCCCCGGCGCGTGCGGGTGGCGGAACCCCTAAAAAACCCAAAACCGACCCGCATTGAACCGCcact < 2:424915/89‑1 (MQ=255) tttGCCGGGGAGATGTCCCCCCGGCGCGTATTGGTGGCCGGACCTCTAAAAACCGCAAAAACGCTCCGCTTTGAACCGCcactgacactg < 1:462789/90‑1 (MQ=255) tttGCCAGGGAATTGTCCCGCCGGCGCGTCCGGGTGGCCGGACCGCTAAATACCGCAAAAACGACCCGCATGGAACCGCcactgacactg < 1:89696/90‑1 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 1:294773/1‑90 (MQ=255) agttGTCCCGCCGGCGCGGACTGGTGGCCGGACCGCTAAAAACCCCAAAAACGCCCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:164142/87‑1 (MQ=255) agaTGTCCCCCCGGCGCGTACGGGGGGCCGGAACCCAAAAAAACCCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 2:312088/90‑1 (MQ=255) aTGTCCCGCCCGCGCGTCCTGGCGGCCGGAACCCTAAAAACCCCAAAAACGACCCGCATTAACCCGCCACTGACACTGACCATTGAACAg < 2:2057/90‑1 (MQ=255) tCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGtgtg > 1:391901/1‑90 (MQ=255) ccAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa > 1:336183/1‑90 (MQ=255) | ATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCA > NZ_CP009273/3214029‑3214206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |