Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273673,6070CA54.5% 4.2 / 13.3 11T246N (ACT→AAT) djlBJ domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCG  >  NZ_CP009273/673524‑673654
                                                                                   |                                               
gCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAAcc                                           <  2:227410/90‑1 (MQ=255)
     gAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGGAAATGAAACTGATa                                      >  1:517700/1‑90 (MQ=255)
                               ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGGAAATGAAACTGATAATGAAAAAGCGCCCTATTTTTAATaa            >  2:119147/1‑89 (MQ=255)
                               ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGGAAAAGAAACCGATAAATAAGATGCGCCCTATTTTTAATaa            >  2:404588/1‑89 (MQ=255)
                               ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTTTTATTAATAc            >  1:219110/1‑90 (MQ=255)
                               ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAATGAACCTGGTAATGAAGATGCGGCCTATTATGAATAc            >  1:71673/1‑90 (MQ=255)
                               ggTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAAAAGAAACCCATAATAAAGATGAGCCCTTTTTTTAATaa            >  2:544038/1‑89 (MQ=255)
                                    ccTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGGAAATGAACCTGATAATTAAAAAGCGCCCTATTTTTAAAAAGCGCa       >  2:98845/1‑90 (MQ=255)
                                      tGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAAc     <  1:98845/90‑1 (MQ=255)
                                         atatCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAAcgcg  <  1:257398/90‑1 (MQ=255)
                                         atatCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAAcgcg  <  1:41113/90‑1 (MQ=255)
                                                                                   |                                               
GCACAGAAATTTACTCCGCTGGTATAGCGCAGGTCGCCTGGATATCCCCGAACTTCTGGATTACGCCCAGTCATGGCGGGAAACTGAACCTGATAATGAAGATGCGCCTTATTATGAATACGCGCAACGCG  >  NZ_CP009273/673524‑673654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑