Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG54.5% 6.3 / 10.3 11T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822875‑823025
                                                                                |                                                                      
aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggggg                                                               >  2:320646/1‑88 (MQ=255)
                    ccATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgt                                           <  2:465059/90‑1 (MQ=255)
                              gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAg                                 >  1:25413/1‑90 (MQ=255)
                                         ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt                      <  1:48260/90‑1 (MQ=255)
                                              aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc                 <  2:206627/90‑1 (MQ=255)
                                              aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTTATGc                 >  1:52365/1‑90 (MQ=255)
                                                gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCAt               >  1:186274/1‑90 (MQ=255)
                                                     tGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAACCCGGAAGGGGGTTCGTCGGGAAAAAAAAAGTCCGGGTCATGCATCAACg          >  1:484313/1‑90 (MQ=255)
                                                        tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGaaa       >  1:495127/1‑89 (MQ=255)
                                                        tgtgCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTTGTCGGGAAACAGAAAGTCCGGGTCATGGATTAGCgcaa       >  1:339665/1‑87 (MQ=255)
                                                           gCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgg    >  1:122009/1‑90 (MQ=255)
                                                             aGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc  <  1:375313/90‑1 (MQ=255)
                                                                                |                                                                      
ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822875‑823025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑