| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,229,859 | 0 | A | C | 60.0% | 0.1 / 10.8 | 15 | G105G (GGT→GGG) | nhaB | Na(+)/H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (0/9); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGAT > NZ_CP009273/1229780‑1229945 | gCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGccacca > 1:249939/1‑90 (MQ=255) aCAGCAGCATTTTGGAGCGAATGCTTAACAGAAACCGGGAAAATATGAACAGAAACAGCTGTTTCAAAAAATAGATCCCCGCCACCATaa < 2:344997/90‑1 (MQ=255) agcaTTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATCCCCGCCACCATAAACATc < 2:288856/90‑1 (MQ=255) cGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTgccgcc < 1:172497/90‑1 (MQ=255) tAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTa > 1:309459/1‑60 (MQ=255) tAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTa < 2:309459/60‑1 (MQ=255) tGAACAGCAACAGCTGTTTCATAAAATAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCAc < 2:464094/90‑1 (MQ=255) tGAACAGCAACAGCTGTTTCAAAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCAc < 1:288120/90‑1 (MQ=255) agcaacagcTGTTTCATAAAATAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACg < 2:238514/90‑1 (MQ=255) agcaacagcTGTTTCATAAAATAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACg < 2:425967/90‑1 (MQ=255) tGTTTCATAAAATAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCcgcg < 1:463242/90‑1 (MQ=255) aaTAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGc < 1:33162/90‑1 (MQ=255) aaTAGATCCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGc < 2:182772/90‑1 (MQ=255) aGATCCCCGCCACCATAAACATCAGTAACAGCAAGCCTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGa < 2:249939/90‑1 (MQ=255) gATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGAt > 1:345778/1‑90 (MQ=255) | GCGACAGCAGCATTTTGGAGCGAATGCTTAACAGCAAACGGGTAAATATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGAT > NZ_CP009273/1229780‑1229945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |