Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,905 T→G L28R (CTC→CGC)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9050TG96.9% 95.6 / NA 32L28R (CTC→CGC) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (13/18);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49821‑49991
                                                                                    |                                                                                      
cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   >  1:21884/1‑90 (MQ=255)
 aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTg                                                                                  <  1:80154/90‑1 (MQ=255)
      tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                                                                             <  2:101737/90‑1 (MQ=255)
      tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                                                                             <  2:36319/90‑1 (MQ=255)
      tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                                                                             >  1:36319/1‑90 (MQ=255)
       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa                                                                            >  2:73209/1‑90 (MQ=255)
       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa                                                                            >  2:330651/1‑90 (MQ=255)
         gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAAc                                                                          <  2:184622/90‑1 (MQ=255)
                  cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt                                                                 <  1:330651/90‑1 (MQ=255)
                   ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:193025/1‑90 (MQ=255)
                   ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:300707/1‑90 (MQ=255)
                   ggcgTTAGCGGGAGCACGCGTTATCGGCATGGAAAACGCCCCGCGGTGTATGCGGCCTTCCCATCTCTCCCGGTTTAATAGCAAGTCCtt                                                                >  2:183140/1‑90 (MQ=255)
                       ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaat                                                            >  1:263350/1‑90 (MQ=255)
                       ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaat                                                            <  2:302238/90‑1 (MQ=255)
                               aGATCGCGTTATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  2:543552/90‑1 (MQ=255)
                               aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  1:5015/90‑1 (MQ=255)
                                    gcgTTATCGGCATGGAAAACGCCATGCCGTGGGACCTGCCTGCCGATCGCGCCTGGTTTTAACGCAACACCTTAAATAAAACCGGGATTa                                               >  2:315644/1‑90 (MQ=255)
                                    gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  2:233068/1‑90 (MQ=255)
                                       ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            <  1:395478/90‑1 (MQ=255)
                                       ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            >  1:24746/1‑90 (MQ=255)
                                       ttATCGGCAGGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            <  2:366604/90‑1 (MQ=255)
                                        tATCGGCATGAAAAACGCCATGCCGGGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  2:152719/90‑1 (MQ=255)
                                        tATCGGCAGGAAAACCGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  2:92606/90‑1 (MQ=255)
                                          tCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                         <  1:73209/90‑1 (MQ=255)
                                            ggCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                       <  2:258768/90‑1 (MQ=255)
                                                   aaaaCGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                <  1:471050/90‑1 (MQ=255)
                                                                gTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                   >  1:20843/1‑90 (MQ=255)
                                                                      cctgcctgCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc             <  1:376620/90‑1 (MQ=255)
                                                                        tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt           >  1:190471/1‑90 (MQ=255)
                                                                             gccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGACTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa      >  1:460085/1‑90 (MQ=255)
                                                                                 aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:21884/90‑1 (MQ=255)
                                                                                 aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  1:383242/90‑1 (MQ=255)
                                                                                    |                                                                                      
CAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49821‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: