| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,033 | 0 | G | T | 61.5% | 4.6 / 18.0 | 13 | A5S (GCA→TCA) | ybbY | uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base T (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTC > NZ_CP009273/535957‑536105 | agcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGa < 1:379536/90‑1 (MQ=255) cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa < 2:428771/90‑1 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGGTTTTTTTCAATTTTTCAGTCAGCCGCGCAAGCCTGtttt > 1:667933/1‑88 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTTTTTTCAATTTTTCAGTCCGCCCCGAAAGCCTGTTTTCAggg > 1:677455/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTAAGGTGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAACCCGTTTTCAGGa > 1:154090/1‑90 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGGGTGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAggg > 2:499450/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGGGTGGTTTTTTTCAATTTTTCAGGCCGCCGCGGAAACCTGTTATCAggg > 1:671687/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAggg > 1:110178/1‑89 (MQ=255) gCCCGTCCTTGCGGCGGGCATTTTCCCGGTAAGGTGGGTTTATGTTCCATTTTTCAGTCAGCCGCGAAAGCCTGTTATTAGGGTTTTAgg > 1:407112/1‑89 (MQ=255) gggCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg > 1:272121/1‑39 (MQ=255) gggCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg < 2:272121/39‑1 (MQ=255) gggCATTCTCCGGGTAAGGGGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTTAGTGGGtttttttttttt > 1:219705/1‑90 (MQ=255) ttCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGc < 2:146801/90‑1 (MQ=255) gtgtgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTgg < 2:219705/90‑1 (MQ=255) tgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtc < 1:328085/90‑1 (MQ=255) | AGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTC > NZ_CP009273/535957‑536105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |