| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 64.3% | ‑3.4 / 13.7 | 14 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (1/4); new base C (9/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760098‑760270 | ggTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCg < 2:163669/90‑1 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGAc < 2:657012/90‑1 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCCTCCCCGGCCCTTACTTTTTTGAc > 2:737672/1‑90 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCCCCCCCGGCGCCAACTTTTTTGAc > 1:715504/1‑90 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCCGGGTCTAACTTTTTTGACAt > 2:40885/1‑90 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCAGCCCCGGGGCTTACTTTTTTGACAt > 1:311926/1‑90 (MQ=255) gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGGTCTAACTTTTTCGACATTTTCGGAAt > 2:544862/1‑90 (MQ=255) gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCCTCCCCGGGCCTCACTTTTTTGACATTTTCCAAAt > 1:565434/1‑90 (MQ=255) tCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGc < 1:737672/90‑1 (MQ=255) gTCGACCTGTGTCGGTATCGGCGGGGACCCGCTCCCCGGGTCTTACTTTTTTGACATTTTTGGAAAGTTTGAAAAAAAACCGCCGAccca > 1:203582/1‑88 (MQ=255) ccTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGa < 1:425937/90‑1 (MQ=255) ggTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTCGACATTTTCGAAAAGTTTGAAAAAAAACCCCCGACCGAAGCGATCGtgatg > 1:197016/1‑90 (MQ=255) tATCGGCGGTGACCCGATCCCCGGCTCTAACTTTTTCGACATTCTCGAAATGTTTGAAAAAAAACCGCAGACCGAAGCGATCGTggtgat > 2:447991/1‑90 (MQ=255) gTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTgaga < 2:368243/90‑1 (MQ=255) gATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg > 2:346193/1‑90 (MQ=255) | GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760098‑760270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |