| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,001,832 | 0 | T | G | 57.1% | 5.7 / 10.7 | 14 | G25G (GGT→GGG) | yedE | selenium metabolism membrane protein YedE/FdhT |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAAC > NZ_CP009273/2001750‑2001920 | cTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATtctct < 2:424341/90‑1 (MQ=255) tgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCtactttct > 2:127344/1‑90 (MQ=255) gtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTa > 1:406760/1‑90 (MQ=255) catgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTTTCTACTTACTAtt > 1:131496/1‑90 (MQ=255) aaCACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCa < 1:199712/90‑1 (MQ=255) aaCACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCa < 1:276588/90‑1 (MQ=255) acGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTTGCATTACTGGCACCt > 1:182353/1‑90 (MQ=255) cGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTTGCATTACTGGCACCtt > 2:564720/1‑90 (MQ=255) tGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTg > 1:233304/1‑90 (MQ=255) aTTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTTGCATTACTGGCACCTTTTTGGCTGTc > 2:509857/1‑90 (MQ=255) aTTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTc > 1:393594/1‑90 (MQ=255) gggCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAAt > 2:674412/1‑90 (MQ=255) cTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAATTTACCCGTTggg > 1:99381/1‑90 (MQ=255) gTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAAc < 2:131496/90‑1 (MQ=255) | CTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAAC > NZ_CP009273/2001750‑2001920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |