Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	481,275	C→A	A20D (GCT→GAT)	acrR →	multidrug efflux transporter transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	481,275	0	C	A	100.0%	35.2 / NA	11	A20D (GCT→GAT)	acrR	multidrug efflux transporter transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/5);  total (6/5)

TAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGC  >  NZ_CP009273/481193‑481351                                                                                   |                                                                             tAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGt                                                                       <  1:575259/90‑1 (MQ=255)  aaCGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTc                                                                      >  1:106932/1‑90 (MQ=255)        tGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTtct                                                                >  2:476379/1‑90 (MQ=255)                   gAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAgggg                                                     >  2:355927/1‑90 (MQ=255)                     aCATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGt                                                   >  2:252775/1‑90 (MQ=255)                      cATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTa                                                  <  1:293890/90‑1 (MQ=255)                      cATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTa                                                  <  2:621218/90‑1 (MQ=255)                                            aagaagCGCAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCgaga                            <  2:188123/90‑1 (MQ=255)                                                   gcAAGAAACGCGCCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCaaa                     >  1:163301/1‑90 (MQ=255)                                                              gcCAACACATCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCg          >  1:206459/1‑90 (MQ=255)                                                                      aTCCTCGATGTGGATCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcgc  <  2:206459/90‑1 (MQ=255)                                                                                   |                                                                             TAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGC  >  NZ_CP009273/481193‑481351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑