| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 350,034 | 0 | T | G | 55.0% | 18.6 / 22.3 | 20 | F540V (TTT→GTT) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/7); new base G (11/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCA > NZ_CP009273/349952‑350122 | gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCAt > 1:375636/1‑90 (MQ=255)gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAATGGGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCAt > 1:364297/1‑90 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGa > 1:112401/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAagag > 1:537633/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTTATTCCGAAagag > 2:5748/1‑90 (MQ=255) cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGGGGGGGGGGGGGGGGTTGTGATTTCGGAagagggg > 2:334486/1‑87 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg > 1:8874/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGGGGGGGCGGTTGTCATTCCGGAAGAGAGCg > 1:110387/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAagagaggg > 1:358452/1‑88 (MQ=255) tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGGGa > 2:51595/1‑90 (MQ=255) tCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGa > 2:45147/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:565698/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:456795/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:8874/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:334486/90‑1 (MQ=255) tGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTGTCCTTCCGAAAGAGAGCGACAGTCTGGa > 1:243989/1‑90 (MQ=255) tGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTTATTTCGAAAGAGAGCGACAGTCTggg > 2:310449/1‑89 (MQ=255) aTGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga < 2:154852/90‑1 (MQ=255) gCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc < 2:407796/90‑1 (MQ=255) gTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCa < 1:301400/90‑1 (MQ=255) | GAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCA > NZ_CP009273/349952‑350122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |