Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0280AC54.5% 6.3 / 12.5 11V556G (GTG→GGG) ybaLKef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCA  >  NZ_CP009273/496941‑497094
                                                                                       |                                                                  
tctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                                                  >  1:123523/1‑90 (MQ=255)
 ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTcacca                                                                 <  2:258389/90‑1 (MQ=37)
           gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc                                                       >  2:544442/1‑90 (MQ=255)
                           gttttATCATCCCTAAATTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc                                       <  1:544442/87‑1 (MQ=255)
                           gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                       >  1:273359/1‑90 (MQ=255)
                                  cATCCCTACATATTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCa                                <  1:99470/90‑1 (MQ=255)
                                         aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt                         >  1:160627/1‑90 (MQ=255)
                                           ttttttttAATTTTTTCCACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc                       <  2:35322/87‑1 (MQ=255)
                                               ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc                   >  2:326083/1‑90 (MQ=255)
                                                  tCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt                >  1:78895/1‑90 (MQ=255)
                                                             gcccGCCACCCACCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc     <  2:259265/88‑1 (MQ=255)
                                                             acccGCCAACCACCTTTTCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTACAGCATGGTACGGGCGATTTCACGCTCGc     <  1:388704/88‑1 (MQ=255)
                                                                cGGCACCCCCCTTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCa  <  2:535736/90‑1 (MQ=255)
                                                                                       |                                                                  
TCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCA  >  NZ_CP009273/496941‑497094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑