Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0310AC54.5% 2.4 / 12.4 11V555G (GTG→GGG) ybaLKef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCA  >  NZ_CP009273/496942‑497094
                                                                                         |                                                               
ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTcacca                                                                 <  2:258389/90‑1 (MQ=37)
          gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc                                                       >  2:544442/1‑90 (MQ=255)
                          gttttATCATCCCTAAATTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc                                       <  1:544442/87‑1 (MQ=255)
                          gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                       >  1:273359/1‑90 (MQ=255)
                                 cATCCCTACATATTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCa                                <  1:99470/90‑1 (MQ=255)
                                        aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt                         >  1:160627/1‑90 (MQ=255)
                                          ttttttttAATTTTTTCCACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc                       <  2:35322/87‑1 (MQ=255)
                                              ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc                   >  2:326083/1‑90 (MQ=255)
                                                 tCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt                >  1:78895/1‑90 (MQ=255)
                                                            gcccGCCACCCACCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc     <  2:259265/88‑1 (MQ=255)
                                                            acccGCCAACCACCTTTTCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTACAGCATGGTACGGGCGATTTCACGCTCGc     <  1:388704/88‑1 (MQ=255)
                                                               cGGCACCCCCCTTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCa  <  2:535736/90‑1 (MQ=255)
                                                                                         |                                                               
CTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCA  >  NZ_CP009273/496942‑497094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑