| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,285 | 0 | A | C | 53.8% | 12.0 / 16.2 | 13 | V18G (GTA→GGA) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAG > NZ_CP009273/1500205‑1500364 | cagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGaa > 2:480044/1‑90 (MQ=255) ccGGCAACAATCGCTGCTTGCCAGATTATTGCCGCTGAACGGGCGTAGCAAATTAATCCCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTgga < 1:466564/90‑1 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTggag > 1:40849/1‑90 (MQ=255) tGCCAGTTTATGGCCGCTGACCGGGGGTAGCAAATAAATCCCGCCAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGc < 2:114365/90‑1 (MQ=255) tGCCAGATTATTGCCGCTGAACGGGCGTAGCCAATTAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGc < 1:230374/90‑1 (MQ=255) gATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGt > 2:203127/1‑90 (MQ=255) gATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGt > 2:543172/1‑90 (MQ=255) ttGCCGCTGAACGGGCGTAGCCAATTAATCCCGCCAGAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCt < 1:143224/90‑1 (MQ=255) gCCGCTGACGGGGCGTAGCAAATAAAGCCCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg < 2:112904/90‑1 (MQ=255) aggggCGTACCAAATAAACCCCGCAAAAAC‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat < 2:51216/87‑1 (MQ=255) ggCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc > 2:10433/1‑90 (MQ=255) aGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa > 1:560973/1‑90 (MQ=255) ccAAATAAACCCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAg < 2:189042/90‑1 (MQ=255) | CAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAA‑CCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAG > NZ_CP009273/1500205‑1500364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |