| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,555,008 | 0 | T | G | 53.8% | 2.1 / 10.2 | 13 | D60A (GAC→GCC) | ddpB | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGA > NZ_CP009273/1554921‑1555092 | aCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTcc < 2:408221/90‑1 (MQ=255) cGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAg > 1:513913/1‑90 (MQ=255) ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGGCAGt > 2:550100/1‑90 (MQ=255) aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCTGGCGAatat > 2:36222/1‑90 (MQ=255) cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGGCAGGCCCAGGTTCTGGGGAAAAtttttc > 2:76096/1‑88 (MQ=255) ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGa < 1:306441/90‑1 (MQ=255) ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCCGTTGCTGGGGAAAATTTTTCAcca > 2:100031/1‑88 (MQ=255) aCCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATAGc > 1:548912/1‑90 (MQ=255) ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACCATAGCa > 1:465774/1‑90 (MQ=255) ggTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcgc > 1:146372/1‑90 (MQ=255) gTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGc > 1:137400/1‑90 (MQ=255) atacaGTGGCTGGTCCAGCCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATc < 1:36222/90‑1 (MQ=255) gCTGGGCCAGTCCCCGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGGCACCCGCCAGTAACCGCGCCGGATCACCAGGGa > 2:377513/1‑90 (MQ=255) | ACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGA > NZ_CP009273/1554921‑1555092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |