| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,598,494 | 0 | C | T | 53.8% | ‑0.5 / 12.4 | 13 | S64L (TCG→TTG) | lsrD | autoinducer 2 ABC transporter permease LsrD |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAT > NZ_CP009273/1598408‑1598572 | gCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGtt < 2:332226/90‑1 (MQ=255) aGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCg < 1:534235/90‑1 (MQ=255) gCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGGTA‑TTTTggttggttttc > 2:281324/1‑87 (MQ=255) aGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCCATA‑TTTTTGTTTGTTGGACCATc > 1:79760/1‑90 (MQ=255) aGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGAGCGGGATTTTTGTTTGG‑TCGGCCATc > 1:73018/1‑90 (MQ=255) cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATA‑TTTTTTTTTTTTCGACCCCCGGccc > 2:315730/1‑89 (MQ=255) cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCcat < 2:101227/90‑1 (MQ=255) cGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTTGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGGGGACTGTTTc > 1:171342/1‑90 (MQ=255) cTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCaa < 2:107230/90‑1 (MQ=255) aaCGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTTGTTTTGTTTGACCATCCGCCCCTGCGCCATTTCATTGGGGGGGCTGTTTTaaaa > 1:554558/1‑89 (MQ=255) aTGGTGATTGTCAGTGGCGGGGTCGATA‑TTTTTTTTTGTTTGACCCCCGGCCCCTTCGCCCTTTCATTGGGGGGACTGTTTCTAAgtggt > 2:557737/1‑90 (MQ=255) aTGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtggt < 1:557737/90‑1 (MQ=255) gATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAt < 1:333496/90‑1 (MQ=255) | GCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATA‑TTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAT > NZ_CP009273/1598408‑1598572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |