| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,778,686 | 0 | T | G | 62.5% | 12.5 / 25.7 | 24 | intergenic (‑316/+435) | BW25113_RS25705/BW25113_RS13825 | DUF5507 domain‑containing protein/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (15/0); total (16/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAC > NZ_CP009273/2778606‑2778774 | aaTTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc < 1:13483/90‑1 (MQ=255) tttATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGGCag > 2:539006/1‑90 (MQ=255) tctcAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGtagtag < 1:539006/90‑1 (MQ=255) ctcAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGa < 1:483783/90‑1 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTTGGCAGTAGTAGATGttttaag > 1:525293/1‑86 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGttttaag > 1:50243/1‑86 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTc < 1:339250/47‑1 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTc > 2:339250/1‑47 (MQ=255) cagcTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCtt < 1:29370/87‑1 (MQ=255) agccTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCtt < 1:444410/88‑1 (MQ=255) ccTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAGGCGGGGCAGAGACATTTTATcccttc > 1:411145/1‑86 (MQ=255) cGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACgg > 2:180976/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGGAGATGTTTTAGGCGTGGCAGAGACATTTTATCCTTTCTCTTCGGc > 1:415803/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGGAGATGTTTAAGGCGGGGGAGAGACATTTTATCCCTTCTCTACGGc > 2:134149/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTGGTCAGTAGTAGATGTTTAAAGCGTGGGGGAGGCAATTTCTCCTTTCTCTatcgg > 2:429741/1‑86 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAGGCGGGGGAGAGACATTTCATCCTTTCTCTACGGCAt > 2:37962/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCAGGAGGAGATGTTTAAAGGGGGGGGGAGAAATTTCATCCGTACTCTACGGccc > 2:444410/1‑88 (MQ=255) cacaAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCAGTAGTAGATGTTTTAGGGGTGGGAGAGACATTTTATCCTTACTCTACGGCAtt > 2:265638/1‑90 (MQ=255) cacaAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCAGTAGTAGATGTTTTAGGCGGGGCAGAGACATTTTATCCTTTCTCTACGGcctg > 2:29370/1‑87 (MQ=255) cATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtacat < 1:35867/90‑1 (MQ=255) aTCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtacata > 1:107617/1‑90 (MQ=255) aTCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGGGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtacata > 1:134314/1‑90 (MQ=255) ctgcctgcGGCGGGGGGTCTGGTCAGTAGGAGATGTTTAAGGCGGGGGGGGGACATTTTATCCTTTCTCTACGGCATTTTTTtacataca > 2:397087/1‑90 (MQ=255) gcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACAtt < 1:180976/90‑1 (MQ=255) gtgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAc < 1:499496/90‑1 (MQ=255) gtgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAc < 2:50243/90‑1 (MQ=255) | AATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAC > NZ_CP009273/2778606‑2778774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |