Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,273,8300AC54.5% 8.1 / 12.7 11T85T (ACA→ACC) agaVPTS N‑acetylgalactosamine transporter subunit IIB
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGG  >  NZ_CP009273/3273741‑3273889
                                                                                         |                                                           
aTCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAaca                                                             >  1:36582/1‑90 (MQ=255)
aTCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAaca                                                             >  1:410048/1‑90 (MQ=255)
        aCGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGa                                                     >  2:473450/1‑90 (MQ=255)
            ttCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTc                                                 >  2:462905/1‑90 (MQ=255)
                   cGCTGAAAAAATTTATCAACAACATTCACCGCGCCGCCGATCGACAAAAAATCCTGCTGGTTTGTAAACCCCCCGCCGATTTCCTGACGc                                          <  1:459611/90‑1 (MQ=255)
                     cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTg                                        >  2:512715/1‑90 (MQ=255)
                              gTTATCAACAACATCCACCGCCCCGCCGACCGACAAAAAATCCTGCTGGTTTGTAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGt                               <  1:76861/90‑1 (MQ=255)
                               ttATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTg                              >  1:113740/1‑90 (MQ=255)
                                             caccgcgCCGCCGTTCGACAAAAAACCCTGCTGGTTTGAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt                <  1:473450/87‑1 (MQ=255)
                                             caccgccCCGCCGATCGAAAAAAATTCCTGTTGGTTTGAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt                <  2:185551/87‑1 (MQ=255)
                                               tCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCg              >  1:467533/1‑90 (MQ=255)
                                                    ccgccgATCGACAGAAAATCCTGCTGTTTTGTAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTa         <  1:228718/90‑1 (MQ=255)
                                                           tcaaCAAAAAATCCTGGTGTTTTGAAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg  <  2:36582/87‑1 (MQ=255)
                                                                                         |                                                           
ATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGG  >  NZ_CP009273/3273741‑3273889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑