| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,357,755 | 0 | A | T | 57.5% | 7.3 / 45.9 | 40 | P530P (CCA→CCT) | yhcD | fimbrial biogenesis outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (11/6); new base T (0/23); total (11/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCA > NZ_CP009273/3357671‑3357842 | ttCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTgg < 2:278249/90‑1 (MQ=255) gggaagcaTCAATTTAGTGCTTAAAAAAATAATGAAGACGAGGGTGTTCTTTTCTACGTCAACATCAGTATCCTTTTTGGAAGAAAAAac < 2:328536/86‑1 (MQ=255) gggCAGCATCACCTTAGTCCTTAAAAAAATAAAAAAGACGATGGTGATCTTTTCTACGTAAACATCAGTATTCCTTTTGGAAGACAAAac < 2:345431/89‑1 (MQ=255) cagcaTCAACTTAGTGCTTCAGAAAAAAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACAcc < 1:180249/90‑1 (MQ=255) gcaTCACCTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTCCGTCAACATCATTATTCCATTTGGCAGATCAAACCCCGc < 2:380888/90‑1 (MQ=255) gcaTCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTAAACATCAGTATTCCATTTGGCAGATAAAACACCGc < 2:171030/90‑1 (MQ=255) gcaTCAACTTAGTGCTTAAAAAAATAATGAAGCCGATGGTGATCTGTTCTACGCCAACATCAGTATTCCTTTTGGCAGATCAACCACCGc < 2:169972/90‑1 (MQ=255) aTCAACTAAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTCCGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCca < 2:63748/90‑1 (MQ=255) tCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCcac < 1:63947/90‑1 (MQ=255) tCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACCCCGCcac < 2:427303/90‑1 (MQ=255) tCAACTTAGTGCTTAAAAAAAAAATGAAGACGATGGTGATCTTTTCTACGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGCcac < 2:67899/90‑1 (MQ=255) aaCTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCacac < 1:36565/90‑1 (MQ=255) aaCTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCacac > 2:68858/1‑90 (MQ=255) aaCTTAGTGCTTCAGAAAATAATGAAGACGAGGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGCCacac < 1:557385/90‑1 (MQ=255) aaCTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTTTTCTACGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGCCacac < 2:365648/90‑1 (MQ=255) aaCTTAGTGCTTAAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCacac < 2:151269/90‑1 (MQ=255) aaCTTAGTCCTTAAAAAAAAAATGAAGACGATGGTGATTTGTTCTCCGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCacac < 2:30551/90‑1 (MQ=255) aCTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCACACt < 2:30498/90‑1 (MQ=255) cTTAGTGCTTAAGAAAAAAATGAAGACGATGGTGCTCTTTTCTCCGTCAACACCATTATTCATTTTGGCAAACCAACCACCGCCACACTg < 2:344433/90‑1 (MQ=255) gTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGtata > 2:269658/1‑90 (MQ=255) gTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGtata > 1:412747/1‑90 (MQ=255) tgtttCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTAAACATCAGTTTTCCTTTTGGCAGATAAAACCCCGCCACACTGTATAc < 2:111307/87‑1 (MQ=255) aaaTAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGa > 2:97128/1‑90 (MQ=255) ataataAAGACGAGGGTGATTTGTTCTCCGTCAACATCATTATTCCTTTTGGCAAATCAAACACCGCCACACTGTATACTCGCCATGACg < 2:86911/90‑1 (MQ=255) aaaatAAAGAGGATGGTGTTTTTTTCTCCGCAAACACCAGTATTCTTTTTGGAAAAAAAAACCCCGCCACACTGTATACTCGCCATGACg < 2:564266/88‑1 (MQ=255) aTGGTGTTCTGTTCTACGTAAACATCAGTATTCCTTTTGGAAAACCAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAAccc < 2:295095/90‑1 (MQ=255) gggTGATCTTTTCTACGTCAACATCAGTTTTCCTTTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCa < 1:557900/89‑1 (MQ=255) gATCTTTTCTACGCCAACATCAGTATCCCTTTTGGCAGATAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTAt < 1:550865/90‑1 (MQ=255) tttCTACGTCAAAATCATTATTCCTTTTGGCAGACAAACCACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc < 1:381658/89‑1 (MQ=255) gTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc > 2:212622/1‑90 (MQ=255) gTTCTACGCCAACATCAGTATTCCTTTTGGCAAATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc < 2:239251/90‑1 (MQ=255) tCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTg > 2:6457/1‑90 (MQ=255) ccGTAAACATCAGTTTTCTTTTTGGAAGAAAAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg < 1:323722/89‑1 (MQ=255) acatCAACAATAGTGTCCCTTTTGACAGAGCAACCTCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg < 2:207496/87‑1 (MQ=255) aCGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg > 2:400270/1‑90 (MQ=255) aCGCCAAAATCGGTTTTCCTTTTGGCAACTAAATCACCGCCCCACTGTATACTCGCCATGACGATCATAAAACCCCCTATGGTACTGgtg < 2:388484/90‑1 (MQ=255) aaCATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATg > 2:485255/1‑90 (MQ=255) gTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtcg > 2:72886/1‑90 (MQ=255) gTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtcg > 1:154264/1‑90 (MQ=255) ccATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCa > 1:215982/1‑90 (MQ=255) | TTCCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCA > NZ_CP009273/3357671‑3357842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |