| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,837,579 | 0 | T | G | 60.0% | 9.0 / 15.8 | 15 | F86V (TTT→GTT) | adeD | adenine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (9/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACC > NZ_CP009273/3837497‑3837665 | cATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGGGCCCGGGGTTTTTGa > 2:30953/1‑90 (MQ=255) gCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGGGCCAGGGGTTATTTATggt > 2:531575/1‑88 (MQ=255) ccGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTc < 2:78771/90‑1 (MQ=255) tACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATcc < 2:398987/90‑1 (MQ=255) tGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTTAATCCCGCat > 1:349349/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCCGGGGTTTTTTATGCTCACCTGCATATTGAATCCAGCatga > 2:497257/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTTTTTATGCTCACCCGCATATTGAATCCAGCatga > 2:547796/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAGCatga > 1:147049/1‑90 (MQ=255) gCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatgatg > 2:497395/1‑90 (MQ=255) gCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTTATGCTCACCTGCATATTGAATCCAGCatgatg > 2:557415/1‑90 (MQ=255) ggCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGcc > 2:105942/1‑90 (MQ=255) tGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGc < 1:497395/90‑1 (MQ=255) cgcgGCGCAACGGCGGGGCCGGGGGTTATTGATGCTCACCGGCGTATTTAATCCGGCATGGTAACGCCGGGCACTTTTGAAACCCCGAcc > 2:446219/1‑90 (MQ=255) cAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTgacga < 1:379179/90‑1 (MQ=255) gggTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGAcc > 1:23073/1‑90 (MQ=255) | CATTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCCTGACGACC > NZ_CP009273/3837497‑3837665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |