Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9990TG61.5% 4.1 / 28.9 26V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/9);  new base G (16/0);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 3.20e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-06
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGA  >  NZ_CP009273/349917‑350065
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gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                             <  1:554948/90‑1 (MQ=255)
gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGaa                                                             >  1:288249/1‑89 (MQ=255)
        tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                     <  2:29898/90‑1 (MQ=255)
         cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa                                                    <  1:151271/90‑1 (MQ=255)
             tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                <  1:139193/90‑1 (MQ=255)
             tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                <  2:177786/90‑1 (MQ=255)
              ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGTAAGAGGGGGTGGAAAGGCa                                               >  1:535602/1‑90 (MQ=255)
                     cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTGAAAGGGGAggggggg                                        >  1:326552/1‑88 (MQ=255)
                     cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAgggggcg                                        >  1:130136/1‑90 (MQ=255)
                                   gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCAt                          >  1:375636/1‑90 (MQ=255)
                                   gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAATGGGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCAt                          >  1:364297/1‑90 (MQ=255)
                                        tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGa                     >  1:112401/1‑90 (MQ=255)
                                             aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAagag                >  1:537633/1‑90 (MQ=255)
                                             aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTTATTCCGAAagag                >  2:5748/1‑90 (MQ=255)
                                                cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGGGGGGGGGGGGGGGGTTGTGATTTCGGAagagggg             >  2:334486/1‑87 (MQ=255)
                                                 aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg            >  1:8874/1‑90 (MQ=255)
                                                 aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGGGGGGGCGGTTGTCATTCCGGAAGAGAGCg            >  1:110387/1‑90 (MQ=255)
                                                 aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAagagaggg            >  1:358452/1‑88 (MQ=255)
                                                  tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGGGa           >  2:51595/1‑90 (MQ=255)
                                                  tCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGa           >  2:45147/1‑90 (MQ=255)
                                                   ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc          <  1:565698/90‑1 (MQ=255)
                                                   ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc          <  2:456795/90‑1 (MQ=255)
                                                   ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc          <  2:8874/90‑1 (MQ=255)
                                                     gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg        <  1:334486/90‑1 (MQ=255)
                                                           tGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGTTTGTCCTTCCGAAAGAGAGCGACAGTCTGGa  >  1:243989/1‑90 (MQ=255)
                                                           tGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTTATTTCGAAAGAGAGCGACAGTCTggg  >  2:310449/1‑89 (MQ=255)
                                                                                  |                                                                  
GCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGA  >  NZ_CP009273/349917‑350065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑