Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,905	T→G	L28R (CTC→CGC)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,905	0	T	G	100.0%	107.0 / NA	35	L28R (CTC→CGC)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/22);  total (13/22)

CTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49819‑49991                                                                                       |                                                                                       ctcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCgcgc                                                                                     <  1:498971/90‑1 (MQ=255)  tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:255593/90‑1 (MQ=255)   cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   <  2:368955/90‑1 (MQ=255)   cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   <  2:186915/90‑1 (MQ=255)   cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   <  1:466404/90‑1 (MQ=255)   cAATGATCAGTCGGATTGCGGCGTTAGCGGTAGATCGCGGTATGGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCt                                                                                   <  2:523606/90‑1 (MQ=255)          cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa                                                                            >  2:472709/1‑90 (MQ=255)           aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                                           >  1:484242/1‑90 (MQ=255)                gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa                                                                      <  1:472709/90‑1 (MQ=255)                 aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac                                                                     >  2:435242/1‑90 (MQ=255)                     cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt                                                                 <  2:91437/90‑1 (MQ=255)                      ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAGACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  1:292062/1‑90 (MQ=255)                      ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:521910/1‑90 (MQ=255)                      ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  1:67946/1‑90 (MQ=255)                      ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                                                >  2:4370/1‑90 (MQ=255)                      ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCGCGCCTGGGTTAAACGCAACACCtt                                                                >  2:504006/1‑90 (MQ=255)                           tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    <  1:431381/65‑1 (MQ=255)                           tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGcc                                                                                    >  2:431381/1‑65 (MQ=255)                                gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                                      <  2:484242/90‑1 (MQ=255)                                  aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTACATAAACCCGt                                                    <  2:504889/90‑1 (MQ=255)                                  aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  1:116152/90‑1 (MQ=255)                                       gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGGTTAAACGCAACACCTTAAATAAAACCGTGATTa                                               >  2:402824/1‑90 (MQ=255)                                           tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  1:87609/90‑1 (MQ=255)                                               ggCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                       <  1:559319/90‑1 (MQ=255)                                                 cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                     <  2:296513/90‑1 (MQ=255)                                                  aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  2:215048/90‑1 (MQ=255)                                                           gCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaa                           <  1:184834/90‑1 (MQ=255)                                                               tGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    <  1:569384/77‑1 (MQ=255)                                                               tGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    >  2:569384/1‑77 (MQ=255)                                                                     ggACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  1:521910/90‑1 (MQ=255)                                                                        aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc              >  1:385327/1‑90 (MQ=255)                                                                           tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                     >  1:84756/1‑80 (MQ=255)                                                                           tgcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                     <  2:84756/80‑1 (MQ=255)                                                                                    aTCGCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:287341/90‑1 (MQ=255)                                                                                    aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  1:435242/90‑1 (MQ=255)                                                                                    aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  1:1156/90‑1 (MQ=255)                                                                                       |                                                                                       CTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49819‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑