Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,269,479	T→C	V243A (GTC→GCC)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,269,479	0	T	C	100.0%	81.9 / NA	25	V243A (GTC→GCC)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

AGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTA  >  NZ_CP009273/2269400‑2269562                                                                                |                                                                                    aGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGc                                                                           <  2:419930/90‑1 (MQ=255) aGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGc                                                                           <  2:394722/90‑1 (MQ=255) aGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGc                                                                           <  2:469323/90‑1 (MQ=255)     tGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTa                                                                       <  2:83644/90‑1 (MQ=255)      ggTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTat                                                                      >  1:52492/1‑90 (MQ=255)             gtggtgCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTg                                                               <  1:150607/90‑1 (MQ=255)                     ggcggAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCatat                                                       >  1:469322/1‑90 (MQ=255)                       cggAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGt                                                     <  2:469322/90‑1 (MQ=255)                         gAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTg                                                   >  1:49216/1‑90 (MQ=255)                           gTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGcc                                                 >  2:459969/1‑90 (MQ=255)                             ttttACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTa                                               <  2:52492/90‑1 (MQ=255)                               ttACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAAt                                             >  2:411572/1‑90 (MQ=255)                                   gTACTCGTAATTTCGAGTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCa                                         >  2:431708/1‑90 (MQ=255)                                   gTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCa                                         >  2:479140/1‑90 (MQ=255)                                    ttcTCGTACTTTCGACTACATTCGTGCGGCACCGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCAt                                        <  2:294965/88‑1 (MQ=255)                                    tACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCAt                                        <  2:49216/90‑1 (MQ=255)                                     aCTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATg                                       >  2:364557/1‑90 (MQ=255)                                                 gACTACATTCGTGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTc                           <  2:248567/90‑1 (MQ=255)                                                            tGCGGCACAGGCGCTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTAcc                >  2:524546/1‑90 (MQ=255)                                                                        gcTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATg    >  1:375783/1‑90 (MQ=255)                                                                         cTTGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGt   <  1:364557/90‑1 (MQ=255)                                                                          ttGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGt                                                     <  1:223028/39‑1 (MQ=39)                                                                          ttGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGt                                                     >  2:223028/1‑39 (MQ=39)                                                                          ttGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTa  >  2:270957/1‑90 (MQ=255)                                                                          ttGGCGCCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTa  >  2:92850/1‑90 (MQ=255)                                                                                |                                                                                    AGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTA  >  NZ_CP009273/2269400‑2269562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑