| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 624,108 | 0 | T | G | 53.8% | 7.9 / 12.7 | 14 | G34G (GGT→GGG) | entA | 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase EntA |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGG > NZ_CP009273/624033‑624181 | ggTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGtt < 2:750619/90‑1 (MQ=255) cgcAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGa < 2:544593/90‑1 (MQ=255) tATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGGGGGGAAGGTACAGGGTTTGGTCACACGGGCACTCCGGGGCAATATcccct > 2:349613/1‑88 (MQ=255) tATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGG‑TTTGATCAAGCGTTCACTCAGGAGCAATATCCCttt < 2:568201/90‑1 (MQ=255) tCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGGGGGGAAGGTAAAAGGTTTGATCAAGCGGTTACTCCGGGGCAATATCCCCTTg > 1:371540/1‑90 (MQ=255) tCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGGGCGAAAGGTAAAGGGTTTTTTCAAGGGGTCACTCAGGGGGAATATTCCCTTg > 1:423236/1‑90 (MQ=255) tCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGGGGAAGTTACAGGGTTTGGTCGAGGGGTGACTTAGGAGGAAAAACCCTTTg > 2:547429/1‑90 (MQ=255) tCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGttt < 1:268790/55‑1 (MQ=255) tCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGttt > 2:268790/1‑55 (MQ=255) aCGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGGGAAAGTTACAGGGTTTGATCAAGCGTTTACTCAGGGGCAATATCCCCTTTCGACCg > 1:237534/1‑90 (MQ=255) cGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGGTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTTCGGCCGGAGTga > 2:470886/1‑90 (MQ=255) cGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTAAAGGGTTTGATCAAGCGTTCACTCAGGGGCCAAATCCCTTTTCGAGCGAAGTgg > 1:601185/1‑89 (MQ=255) tttGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGcc < 2:237534/90‑1 (MQ=255) cGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTgcgc < 2:57204/90‑1 (MQ=255) ggAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAg < 2:417633/90‑1 (MQ=255) gAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAgg > 2:716845/1‑90 (MQ=255) | GGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGG > NZ_CP009273/624033‑624181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |