| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 765,675 | 0 | A | C | 61.1% | 8.3 / 14.3 | 18 | T748P (ACC→CCC) | mngB | mannosylglycerate hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/11); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT > NZ_CP009273/765614‑765756 | gCCAGTCCCGGGCTCACAACTACGTGGTCTGCTTTCTTGTCCCCTAAGTTTATTGAGTTATCCCGGTACGCAAACCGCCGCTGGTGTAGc < 2:600748/90‑1 (MQ=255) cacaACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAg > 2:243072/1‑90 (MQ=255) tACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGc > 1:150184/1‑90 (MQ=255) gggggTCTGTTTTGTGGTGGCCTAAGTTTTTGGATTTAGCCAGGTTCGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:402905/87‑1 (MQ=255) cggggTCTGTTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:104358/87‑1 (MQ=255) cGTGGTCTGTTTTTTTGTCGCCTAAGTTTATTGAGTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:101044/90‑1 (MQ=255) cGTGGCCTGTTTTCTGGTCGCTAAGGTTTATTGGGTTACCCGGGCACCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:564322/90‑1 (MQ=255) tGGTCTTTTTTTTTGTCGCCTAATTTTTTTGATTTATCCCGGTACCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGAc < 1:243072/90‑1 (MQ=255) tGGTCTGCTTTCTTGTCCCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGAc < 2:113114/90‑1 (MQ=255) gTCTGCTTTCTTGTCGCCTAATTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc < 2:175652/90‑1 (MQ=255) gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg > 1:174433/1‑86 (MQ=255) gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 2:174433/86‑1 (MQ=255) cTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCa > 2:410291/1‑90 (MQ=255) tttGTCGCTTAATTTTATTGATTTATCCCGGTCCGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAgt < 1:316259/89‑1 (MQ=255) cGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac > 2:726005/1‑90 (MQ=255) aattttATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTAcaacaa < 2:162483/87‑1 (MQ=255) gTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACaaaa < 2:86496/90‑1 (MQ=255) tgcgTTATACGGGTAAGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGCGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAt < 2:407940/87‑1 (MQ=255) | GCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAT > NZ_CP009273/765614‑765756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |