| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,074,938 | 0 | C | G | 53.8% | 12.7 / 17.8 | 13 | L60V (CTG→GTG) | putP | sodium/proline symporter PutP |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATT > NZ_CP009273/1074858‑1075024 | cagacTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCT‑GTTAATgg < 1:454510/88‑1 (MQ=255) atTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCg < 2:331030/90‑1 (MQ=255) gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATTTGGGGCGGGGT‑GTTTATGGGGGTGGCggggcg > 2:454510/1‑87 (MQ=255) gTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGCTGGGG‑GTTTATTGGGGTGCCGGGGG‑CTGgtt > 2:392295/1‑90 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTGTGAGGGGGGGGGTGGTTATTGGGGTTTGGGGGGG‑GGGTTTTTTTTTCCg > 1:701561/1‑90 (MQ=255) tGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGCTGGGT‑GTTTATGGGGGTGGCGGGGGCCGGTTTTTTTTTCCg > 2:38812/1‑90 (MQ=255) aTTCGTGACGGCATTATCGGCGGGGGGGTCGGGGATGAGGGGGTGGGT‑GTTTTTGGGGGGGGCGGGGG‑GGGTTTTTTTTTTTGGGGtttt > 2:491208/1‑89 (MQ=255) aTTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGGCGGGGG‑GTTTATGGGGGTTCCGGGGG‑GGGTTTTTTTTTTCGGGGtttt > 2:593414/1‑89 (MQ=255) cATTATCGGCGGGTGCGTCGGGTATGAGCGGGTGGGT‑GTTTATGGGGGTGGCGGGGG‑CTGTTTTTTTTTTCGGGGTTTTCGGAAACTGGa > 2:141271/1‑90 (MQ=255) ttATCGGCGGGTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATc < 1:666182/90‑1 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 1:528802/90‑1 (MQ=255) gTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGcc < 1:649211/90‑1 (MQ=255) gCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTg < 2:116517/90‑1 (MQ=255) tggct‑gTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGAtt < 1:239035/90‑1 (MQ=255) | ACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCT‑GTTAATGGGGTTGCCGGGCG‑CTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATT > NZ_CP009273/1074858‑1075024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |